Laboratory of Nanomedicine And Histotechnology

Dedicated to projects related to the pathophysiological mechanisms of disease and new therapeutic and diagnostic alternatives using nanomedicine techniques, nanoparticle tracking, histology, immunohistochemistry, immunofluorescence and confocal microscopy.
Structure: nanoparticle tracking system - NanoSight -, tissue embedding station, microtome, cryostat, grid fluorescence microscope and confocal microscope.

Coordinator

Team

Daniel Marinowic
Fernando Diz
Fernando Xavier
Simone Salamoni
Allan Alcará
Daniela Abreu
Fernanda Bruzzo
Me. Isadora Ghilardi
Paula Gabrielli dos Santos
Felipe Krimberg
Fernanda Luccas
Fernando Tonon
Giulia Pinzetta
Laura Provenzi
Livia Menezes
Mariana Bartelle
Nicole Becker
Sabrina Bizotto
Thomás Andrade
Vinicius Wietholter

Phone

+55 (51) 3320-5975

Lines of research

  • Nanomedicine and Extracellular Vesicles
  • Biomarkers and Therapeutic Targets
  • Regenerative Medicine and Advanced Therapies
  • Neurogenetics and Pathogenic Variations
  • Cell Biology and Signaling Mechanisms
  • Pre-clinical and Clinical Epilepsy
  • Epilepsy-associated depression


Main projects

  • Development of Technology for Obtaining Extracellular Vesicles: from biomarkers to therapy
  • Evaluation of the microRNA profiles of extracellular vesicles as a therapeutic target in individuals with mTOR gene mutation
  • Evaluation of the profile of extracellular vesicles in patients with the SCN1A gene mutation and concomitant diagnosis of autism
  • Investigation of prognostic biomarkers and potential therapeutic targets in individuals with the WDR45 gene mutation
  • Pharmacophysiological evaluation of the ionotropic glutamate receptor pathway in individuals with the CDKL5 gene mutation
  • Electrophysiological and molecular evaluation of induced pluripotent stem cells (iPSC) from patients with mutations in the KCNQ2 gene
  • Autologous Bone Marrow Stem Cell Transplantation in drug-refractory temporal lobe epilepsy: Phase II Study
  • Assessment of depression in patients with temporal lobe epilepsy: epigenetic, immunohistological and molecular aspects



Main articles

Stem cell-based therapy for COVID-19 and ARDS: a systematic reviewNpj Regenerative Medicine, v. 6, p. 1-15, 2021. ZANIRATI, G. ; PROVENZI, L. ; LIBERMANN, L. L. ; BIZOTTO, S. C. ; GHILARDI, I. M. ; MARINOWIC, D; SHETTY, A. K. ; DaCosta, J. C..  Click here

Analysis of genes involved in cell proliferation, adhesion, and control of apoptosis during embryonic neurogenesis in Induced Pluripotent Stem Cells (iPSCs) from patients with Focal Cortical DysplasiaBRAIN RESEARCH BULLETIN, v. 155, p. 112-118, 2020. MARINOWIC, D ; MAJOLO, F. ; ZANIRATI, G. G. ; PLENTZ, I. ; Neto, Eliseu Paglioli; Palmini, A. L. F. ; Machado, D. C. ; DaCosta J. C.  Click here

 A Model of Chronic Temporal Lobe Epilepsy Presenting Constantly Rhythmic and Robust Spontaneous Seizures, Co-morbidities and Hippocampal NeuropathologyAging and Disease, v. 10, p. 915, 2019. UPADHYA, D.; KODALI, M.; GITAI, D.; CASTRO, O. W.; ZANIRATI, G.; UPADHYA, R.; ATTALURI, S.; MITRA, E.; SHUAI, B.; HATTIANGADY, B.; SHETTY, A. K.  Click here

Depression comorbidity in epileptic rats is related to brain glucose hypometabolism and hypersynchronicity in the metabolic network architectureEPILEPSIA, v. 59, p. 923-934, 2018. ZANIRATI, GABRIELE ; AZEVEDO, PAMELLA NUNES ; VENTURIN, GIANINA TERIBELE ; Greggio, Samuel ; ALCARÁ, ALLAN MARINHO ; ZIMMER, EDUARDO R. ; FELTES, PAULA KOPSCHINA ; DACOSTA, Jaderson Costa. Click here

Safety and seizure control in patients with mesial temporal lobe epilepsy treated with regional superselective intra-arterial injection of autologous bone marrow mononuclear cellsEngineering and Regenerative Medicine, v. 12, p. 648-656, 2017. Journal of Tissue. DaCosta, Jaderson C.; PORTUGUEZ, MIRNA W. ; MARINOWIC, DANIEL R. ; SCHILLING, LUCAS P. ; TORRES, CAROLINA M. ; DACOSTA, DANIELLE I. ; CARRION, MARIA JÚLIA M. ; RAUPP, EDUARDO F. ; Machado, Denise C. ; SODER, RICARDO B. ; LARDI, SILVIA L. ; GARICOCHEA, BERNARDO. Click here