Evaluation of microglial activation by PET/CT and its association with the appearance of lesions on MRI in patients with relapsing-remitting multiple sclerosis undergoing fingolimode therapy

Project Coordinator: Jefferson Becker, MD, PhD Research Summary: Multiple sclerosis (MS) is the most common inflammatory disease of the central nervous system (CNS), characterized by considerably varying clinical and pathological manifestations. Several pathological studies on Multiple Sclerosis have shown that myelin and its supporting cells, oligodendrocytes, are usually destroyed in MS lesions. White matter lesions ..

Effect of interleukin-1 Beta on the therapeutic potential of bone marrow mononuclear cells in experimental epilepsy

Project Coordinator: Zaquer Suzana Munhoz Costa-Ferro, PhD Research Summary: In this study, we will evaluate the expression of interleukin 1 beta (IL-1β) on LTP after the induction of status epilepticus (SE) and injection of bone marrow mononuclear cells. The specific objectives are: transplant bone marrow mononuclear cells (BMMC) after SE in experimental models; evaluate the ..

Granulocyte colony-stimulating factor (GCSF) treatment in epilepticus rats

Project Coordinator: Zaquer Suzana Munhoz Costa-Ferro, PhD Research Summary: In this study, the effect of G-CSF on spontaneous recurrent seizures (SRS) will be assessed and compared with the effect of bone marrow mononuclear cells (BMMCs). The specific objectives are to treat animals with G-CSF; transplant BMMCs into experimental models of epilepsy; evaluate SRS; evaluate apoptosis, ..

Assessment of the modulation of trophic factor expression after the transplantation of bone marrow mononuclear cells into an animal model with pilocarpine-induced chronic epilepsy

Project Coordinator: Jaderson Costa da Costa, MD, PhD Researcher: Gabriele Goulart Zanirati, MSc (PhD Project) Research Summary: Epilepsy is estimated to affect about 1% of the world population, and approximately 30% of these patients do not respond to drug treatment, becoming refractory. In this scenario, the study and availability of new therapeutic options is essential. ..

Assessment of the permeability of the blood-brain barrier in an experimental model of posterior reversible encephalopathy syndrome through neuroimaging

Project Coordinator: Luiz Carlos Porcello Marrone, MD, MSc (PhD Project in progress) and Jaderson Costa da Costa, MD, PhD Research Summary: The Posterior Reversible Encephalopathy Syndrome (PRES) is a clinical-radiological state characterized by headache, altered level of consciousness, seizures and visual alteration and is associated with a vasogenic edema in the brain white matter, affecting ..

Neurotoxic activity of ureases and derived peptides

Project Coordinator: Célia Regina R. S. Carlini, PhD Research Summary: Ureases are enzymes produced by plants, fungi and bacteria that hydrolyze urea into ammonia and CO2. Besides being toxic for generating ammonia, ureases have other biological properties independent of enzyme action, such as proinflammatory activity and neurotoxicity. Plant ureases have biotechnological potential as biopesticides, and ..

Mechanisms involved in the control of central and peripheral inflammatory response in an experimental epilepsy model with bone marrow stem cell transplant

Research Coordinator: Jaderson Costa da Costa, MD, PhD Research Summary: Epilepsy is a brain disorder characterized by spontaneous and recurring seizures of intense, synchronized, and rhythmic firing of neuronal populations. Pronounced inflammation has been described in epileptogenic brain tissue from drug-resistant patients. Moreover, the change of the blood-brain barrier and increased expression of proinflammatory cytokines ..

Autologous Bone Marrow Stem Cell Transplant in Patients with Temporal Lobe Epilepsy Refractory to Drug Treatment: Phase II Study

Project Coordinator: Jaderson Costa da Costa, MD, PhD Members: Ricardo Vaz Breda/ Daniela Souza de Abreu/ Danielle Irigoyen da Costa/ Li Li Min/ Zaquer Suzana Munhoz Costa/ Fernando Cendes/ Alessandra Marques Pereira/ Carlos Eduardo Silvado/ Eduardo Raupp/ Denise Cantarelli Machado/ André Luis Fernandes Palmini/ Lucas P. Schilling/ Daniel Rodrigo Marinowic/ Marcos Braga Barros/ Ricardo Bernardi ..

Assessment of the Reliability and Reproducibility of Resting-State Functional Magnetic Resonance Images

Project Coordinator: Alexandre Franco, PhD Resting State, or task-free, Functional Magnetic Resonance Imaging (fMRI or rsFMRI) can evaluate the intrinsic connectivity of functional networks in healthy and/or unhealthy brains, suggesting a potential use of this method as a new biomarker for disease monitoring. In the exam, a patient relaxes, lays his/her eyes on a cross ..